| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Peribacillus simplex | TGGTCGATGGAGGACTTTTGG | TGATCTTGGCGTGCTCTGG | 60.00 | 60.08 | 187 | 179 |
100.00%
|
100.00%
|
| Primer 2 | Peribacillus simplex | GGTCGATGGAGGACTTTTGG | TGATCTTGGCGTGCTCTGG | 58.26 | 60.08 | 186 | 179 |
100.00%
|
100.00%
|
| Primer 3 | Peribacillus simplex | TCAGCGTTACTTCGGACAGG | TTAAAGCCGTCCAGCCACC | 59.76 | 60.30 | 285 | 142 |
100.00%
|
100.00%
|
| Primer 4 | Peribacillus simplex | ACAAAGGGTGGGCTGATGC | CCGCTACGAATTGTGCAGC | 60.61 | 59.94 | 243 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Peribacillus simplex | AACGGAGGAAGCTCAGCAG | CCGCTCCGACAATTTCCAC | 59.71 | 59.21 | 215 | 129 |
100.00%
|
100.00%
|
| Primer 6 | Peribacillus simplex | ATCATACCGGATGCGCTCC | CAATGCCTCGACAACCCAC | 59.71 | 59.12 | 238 | 129 |
100.00%
|
100.00%
|
| Primer 7 | Peribacillus simplex | CCGGAAGAAACGAAGCTTGG | ACGGTGCATGTCTCGATGG | 59.48 | 60.15 | 230 | 129 |
100.00%
|
100.00%
|
| Primer 8 | Peribacillus simplex | CGCTTGAACGCATGTGCA | TGCCGTGCCAAAATCCAC | 59.75 | 58.95 | 185 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Peribacillus simplex | GCCGTGTGAGCCATACATG | TCCTTTAAAACAGCCAGCACA | 58.98 | 58.34 | 214 | 127 |
100.00%
|
100.00%
|
| Primer 10 | Peribacillus simplex | TGCCGAAGAAACCGTTCAAA | TGATCTTGGCGTGCTCTGG | 58.62 | 60.08 | 296 | 127 |
100.00%
|
100.00%
|